Composite

Part:BBa_K4350827:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


NpgA Transcriptional Unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 255
    Illegal XhoI site found at 746
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This transcriptional unit was assembled through golden gate assembly, and all parts required flanking by the appropriate Type IIS restriction and fusion sites.


Source

The sequence for the enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115. The promoter and terminator were acquired from https://doi.org/10.1104/pp.18.01401

References